Add dissociation solution, temperature and time in dissociation_protocol

[arschat]

For which schema is a change/update being suggested?

I would like to request an update to the dissociation_protocol.json schema.

What should the change/update be?

I would like to add new fields - digestion_solution, digestion_time, digestion_time_unit, digestion_temperature - to this schema to allow data contributors to specify the dissociation procedure more precisely.

This update constitutes a miror change to the schema(s) it affects.

What new field(s) need to be changed/added?

digestion_solution

• Field name: digestion_solution
• Field description: Enzyme(s) or reagent(s) of the solution that was used for the digestion of the specimen.
• Field type: array
• Required: no
• Examples: Collagenase; Detergent-based; Liberase
• CV or enum:

Citric-acid based, Detergent-based, Lysis buffer, Accutase, Collagenase, Collagenase A, Collagenase D, Collagenase I, Collagenase II, Collagenase IV, Dispase, Dispase II, DNAse I, Elastase, Hyaluronidase, Liberase, Papain, Protease, TrypLE, Trypsin
List is a combinations of solutions requested among bionetworks & popular dissociation solutions used

digestion_time

• Field name: digestion_time
• Field description: Time of digestion of the specimen.
• Field type: integer
• Required: no
• Examples: 2; 5; 10
• CV or enum: no

digestion_time_unit

• Field name: digestion_time_unit
• Field description: The unit in which the digestion time is expressed.
• Field type: object
• Required: no
• CV or enum: time_unit_ontology

digestion_temperature

• Field name: digestion_temperature
• Field description: Temperature of digestion in Celsius or in general terms (i.e. cold, warm, room temperature).
• Field type: sting
• Required: no
• Examples: 4; 37; cold
• CV or enum: \d+|frozen|cold|room temperature|warm

Why is the change requested?

Lung bionetwork is collecting this information in Tier 2, and other bionetworks are interested in that as well.